3/24/2024 0 Comments Dorsal blastopore lip of embryoa = anterior an = animal bc = blastocoel bp = blastopore d = dorsal dl = dorsal lip dm = deep mesoderm fg = foregut fp = floor plate hg = hindgut l = left no = notochord ov = otic vesicle p = posterior r = right s = somite v = ventral veg = vegetal. (E’,E”) Histological sections (levels indicated in (E)) highlighting expression in the otic vesicle (E’) and nephrostomes ((E”), arrowheads). (E) Staining in the otic vesicle and nephrostomes (arrowheads). Enlargements show multiciliated skin cells ( (D”) cilia indicated by arrowheads) and GRP cells after intercalation into the notochord ( (D”’) arrowheads). (D) Expression at stage 23 in ciliated cells of the epidermis and the floor plate (sagittal section shown in (D’)). (C’) Histological section (plane marked in (C)). The transplanted mesoderm formed a blastopore and moved inside the embryo. Spemann inserted the piece of mesoderm from the first embryo into the second embryo. (C) Expression in the gastrocoel roof plate (GRP) at stage 17 (dorsal explant shown in ventral view). The second embryo had a same-sized piece from the mesodermal area 180 degrees from the dorsal lip. Note that the superficial mesoderm (SM) was free of PACRG mRNA (inset in (B’)). (B’) Sagittal hemisection (plane indicated in (B)) revealing mRNA localization in deep mesoderm. Persistent differences in staining intensities between dorsal and ventral side. Enlargements (boxes) indicate higher expression levels on the dorsal side. Simultaneously, the embryo rotates, the embryonic disk enlarges, and faces upwards. Based on the location of the transplanted cells, what effect would you expect on axis. Elongation of the embryonic disk, notochord and archenteron occur after blastopore closure in an anteroposterior direction marking the anteroposterior and dorsalventral axis of the embryo. Question: In the image below, the dorsal blastopore lip has been transplanted to the ventral region of a host Xenopus embryo. (A’) Sagittal hemisection of embryo shown in (A). This problem has been solved Youll get a detailed solution from a subject matter expert that helps you learn core concepts. Once the dorsal portion of the embryo is established, the movement of the involuting mesoderm establishes the anterior-posterior axis. Transcripts of gsc are present in the dorsal part of the blastopore adjacent to the bottle cells ( Fig. (A) Expression at the four-cell stage (top view). In the normal stage 10 embryo Xbra is expressed in the marginal zone at some distance animalwards off the bottle cell-lined blastopore lip (Fig. PACRG expression during early Xenopus development.
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